Sigma：角质细胞生长因子 人
属性：
产品名称：
角质细胞生长因子 人, KGF, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture
biological source：human
Quality Level：100
Recombinant：expressed in HEK

Sigma：角质细胞生长因子 人

属性：

产品名称：

角质细胞生长因子 人, KGF, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture

biological source：human

Quality Level：100

Recombinant：expressed in HEK 293 cells

Assay：>95% (SDS-PAGE)

Form：lyophilized powder

Potency：4-20 ng/mL EC50

Quality：endotoxin tested

mol wt：dimer 17-30 kDa (glycosylated)

Packaging：pkg of 10 μg

technique(s)：cell culture | mammalian: suitable

Impurities：<1 EU/μg

UniProt accession no.：P21781

storage temp.：−20°C

Gene Information：human ... FGF7(2252)

说明：

General description：

Keratinocyte Growth Factor (KGF) belongs to the fibroblast growth factor 7 (FGF-7) family.[1] It is produced by the mesenchymal cells, γδ T cells, and fibroblasts in several organs.[1][2] The KGF gene is located on the human chromosome at 15q21.2.[3]

Application：

Keratinocyte Growth Factor (KGF) human has been used as a component of the keratinocyte differentiation medium.

Biochem/physiol Actions：

Keratinocyte Growth Factor (KGF) exerts paracrine effects on mesenchymal cells. It exhibits cryoprotective effects on epithelial cells of the lungs, gastrointestinal tract, and the lungs.[1] KGF participates in the activation of IIIb isoform of the fibroblast growth factor (FGF)-receptor 2 (FGFR2-IIIb) present on keratinocytes. This activation further favors proliferation, migration, and survival of keratinocytes.[2]

Physical form：

Lyophilized from a 0.2 μm filtered solution of 1x PBS.

Preparation Note：

HumanKine Keratinocyte Growth Factor (KGF) is expressed as glycosylated 17 and 30 kDa monomers in human HEK 293 cells. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.

Analysis Note：

The activity was determined by the dose-dependent stimulation of the proliferation of 4MBr-5 cells (monkey epithelial cell line).

Legal Information：

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

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